AUTHOR=Liu Suzhen , Luo Houqiang , Wang Meng , Wang Qingyan , Duan Longchuan , Han Qingsong , Sun Siwei , Wei Caixia , Jin Junjie 

TITLE=Microbiome analysis reveals the effects of black soldier fly oil on gut microbiota in pigeon

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.998524

DOI=10.3389/fmicb.2022.998524

ISSN=1664-302X

ABSTRACT=<p>The gut microbiota plays a vital roles in poultry physiology, immunity and metabolism. Black soldier fly oil is known to have a positive effect on the gut microbiota. However, the specific effect of black soldier fly oil on the composition and structure of the gut microbiota of the pigeon is unknown. In this experiment, 16S rDNA high-throughput sequencing was performed to study the effect of different doses of black soldier fly oil on the changes of pigeon intestinal microbes. Results indicated that the different doses of black soldier fly oil had no effect on the gut microbial diversity of the pigeon. Although the dominant phyla (<italic>Proteobacteria</italic>, <italic>Firmicutes</italic>, <italic>Bacteroidetes</italic>, and <italic>Actinobacteria</italic>) and genus (<italic>uncultured_bacterium_f_Lachnospiraceae</italic> and <italic>Desulfovibrio</italic>) in control group and experimental group with different doses were the same, the abundances of some beneficial bacteria (<italic>Megasphaera</italic>, <italic>Intestinimonas</italic>, <italic>Prevotella_9</italic>, <italic>Lachnospiraceae_UCG-001</italic>, <italic>Faecalibacterium</italic>, <italic>Coprococcus_2</italic>, <italic>Parabacteroides</italic>, <italic>Megasphaera</italic>, <italic>Leuconostoc</italic>, <italic>Prevotellaceae_UCG-001</italic>, <italic>Lactococcus</italic>, <italic>Ruminococcaceae_UCG-014</italic>, and <italic>Coprococcus_2</italic>) increased significantly as the concentration of black soldier fly oil increased. Taken together, this study indicated that black soldier fly oil supplementation could improve gut microbial composition and structure by increasing the proportions of beneficial bacteria. Notably, this is the first report on the effects of black soldier fly oil on the gut microbiota of pigeon, which contribute to understanding the positive effects of black soldier fly oil from the gut microbial perspective.</p>