AUTHOR=Zeng Xiang-Yu , Yuan Xiao-Xiao , Peng Ke-Qin , Pan Yin-Tao , Tan Ting-Jun , Wu Nan , Tian Feng-Hua 

TITLE=Taxonomy and control of Trichoderma hymenopellicola sp. nov. responsible for the first green mold disease on Hymenopellis raphanipes

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.991987

DOI=10.3389/fmicb.2022.991987

ISSN=1664-302X

ABSTRACT=<p><italic>Trichoderma</italic> spp. are a group of widespread fungi with important applications in many aspects of human life, but they are also pathogens that cause green mold disease on mushrooms. During a survey of mushroom cultivation in Guizhou, China, five strains of <italic>Trichoderma</italic> from three different localities were isolated from soil in mushroom bags of <italic>Hymenopellis raphanipes</italic>. The typical morphology of having gregarious, reddish stromata and gregarious phialides and the results of phylogenetic analyses based on a combined dataset of RPB2, TEF, and ITS gene sequences demonstrated that these green-spored <italic>Trichoderma</italic> belong to a new taxon, <italic>Trichoderma hymenopellicola</italic>. Pathogenicity tests by covering fungal mycelial blocks or soil mixed with spore suspension in mushroom bags showed similar symptoms to those in the field, and the same fungal pathogen had been observed and re-isolated from these symptoms, which fulfill Koch’s postulates. A primary screening test of nine common fungicides indicated that prochloraz-manganese chloride complex and propiconazole are the top two effective fungicides inhibiting the pathogen, whereas the former was further indicated as a suitable fungicide to control <italic>Trichoderma hymenopellicola</italic>, with a high inhibition ratio to the pathogen and low toxicity to the mushroom.</p>