AUTHOR=Sun Wen-Jing , Wu En-Yun , Zhang Ge-Yin , Xu Bai-Chang , Chen Xiao-Gang , Hao Kai-Yuan , Wang Ying , He Ling-Zhi , Lv Qi-Zhuang TITLE=Total flavonoids of Abrus cantoniensis inhibit CD14/TLR4/NF-κB/MAPK pathway expression and improve gut microbiota disorders to reduce lipopolysaccharide-induced mastitis in mice JOURNAL=Frontiers in Microbiology VOLUME=13 YEAR=2022 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.985529 DOI=10.3389/fmicb.2022.985529 ISSN=1664-302X ABSTRACT=
Established a model of lipopolysaccharide (LPS)-induced mastitis in mice, pathological sections and myeloperoxidase were used to detect the degree of tissue damage, enzyme-linked immunosorbent assay (ELISA) was performed to detect the expression of pro-inflammatory cytokines, meanwhile fluorescence quantitative PCR experiments were performed to detect the mRNA expression of CD14/TLR4/NF-κB/MAPK signalling pathway, and the faeces of mice were collected for 16S measurement of flora. The results showed that