AUTHOR=Zhang Qi , Jia Shiliang , Ding Yicheng , Li Dongmei , Ding Yuting , Zhou Xuxia 

TITLE=Antibacterial activity and mechanism of malondialdehyde against Staphylococcus xylosus and Lactiplantibacillus plantarum isolated from a traditional Chinese dry-cured fish

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.979388

DOI=10.3389/fmicb.2022.979388

ISSN=1664-302X

ABSTRACT=<p>Malondialdehyde (MDA) is one of the most representative reactive carbonyl species (RCSs) produced by lipid oxidation in food. However, the inhibitory effect of MDA on microorganisms has received little attention. Thus, the aim of this study was to reveal the antibacterial mechanism of MDA on <italic>Staphylococcus xylosus</italic> and <italic>Lactiplantibacillus plantarum</italic> isolated from dry-cured fish. The results showed that the minimum inhibitory concentrations (MICs) of MDA on <italic>S. xylosus</italic> and <italic>L. plantarum</italic> were 90 μg/ml and 180 μg/ml, respectively. Time-kill curves indicated a concentration-dependent antibacterial activity of MDA. Moreover, cell wall damage, cell membrane depolarization, intracellular adenosine triphosphate (ATP) decline, Ca<sup>2+</sup> and Mg<sup>2+</sup> leakage, cell morphological destruction and alterations in intracellular biomolecules were observed, which indicated the negative influence of MDA on cell membrane and cellular homeostasis. This study demonstrated the potential antimicrobial properties of MDA and provided theoretical support for the scientific prevention and control of lipid oxidation and microbial contamination in food. This study demonstrated the potential antibacterial properties of MDA and further enriches theoretical studies on the effects of lipid oxidation on microorganisms.</p>