AUTHOR=Zhang Bingzhou , Jiang Changsheng , Cao Hua , Zeng Wei , Ren Jingping , Hu Yaofang , Li Wentao , He Qigai 

TITLE=Transcriptome analysis of heat resistance regulated by quorum sensing system in Glaesserella parasuis

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.968460

DOI=10.3389/fmicb.2022.968460

ISSN=1664-302X

ABSTRACT=<p>The ability of bacteria to resist heat shock allows them to adapt to different environments. In addition, heat shock resistance is known for their virulence. Our previous study showed that the AI-2/luxS quorum sensing system affects the growth characteristics, biofilm formation, and virulence of <italic>Glaesserella parasuis</italic>. The resistance of quorum sensing system deficient <italic>G. parasuis</italic> to heat shock was obviously weaker than that of wild type strain. However, the regulatory mechanism of this phenotype remains unclear. To illustrate the regulatory mechanism by which the quorum sensing system provides resistance to heat shock, the transcriptomes of wild type (GPS2), ΔluxS, and <italic>luxS</italic> complemented (C-luxS) strains were analyzed. Four hundred forty-four differentially expressed genes were identified in quorum sensing system deficient <italic>G. parasuis</italic>, which participated in multiple regulatory pathways. Furthermore, we found that <italic>G. parasuis</italic> regulates the expression of <italic>rseA</italic>, <italic>rpoE</italic>, <italic>rseB</italic>, <italic>degS</italic>, <italic>clpP</italic>, and <italic>htrA</italic> genes to resist heat shock <italic>via</italic> the quorum sensing system. We further confirmed that <italic>rseA</italic> and <italic>rpoE</italic> genes exerted an opposite regulatory effect on heat shock resistance. In conclusion, the findings of this study provide a novel insight into how the quorum sensing system affects the transcriptome of <italic>G. parasuis</italic> and regulates its heat shock resistance property.</p>