AUTHOR=Allen Grace L. , Grahn Ashley K. , Kourentzi Katerina , Willson Richard C. , Waldrop Sean , Guo Jiantao , Kay Brian K. TITLE=Expanding the chemical diversity of M13 bacteriophage JOURNAL=Frontiers in Microbiology VOLUME=13 YEAR=2022 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.961093 DOI=10.3389/fmicb.2022.961093 ISSN=1664-302X ABSTRACT=
Bacteriophage M13 virions are very stable nanoparticles that can be modified by chemical and genetic methods. The capsid proteins can be functionalized in a variety of chemical reactions without loss of particle integrity. In addition, Genetic Code Expansion (GCE) permits the introduction of non-canonical amino acids (ncAAs) into displayed peptides and proteins. The incorporation of ncAAs into phage libraries has led to the discovery of high-affinity binders with low nanomolar dissociation constant (