AUTHOR=Suissa Ronit , Oved Rela , Maan Harsh , Hadad Uzi , Gilhar Omri , Meijler Michael M. , Koren Omry , Kolodkin-Gal Ilana TITLE=Context-dependent differences in the functional responses of Lactobacillaceae strains to fermentable sugars JOURNAL=Frontiers in Microbiology VOLUME=13 YEAR=2022 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.949932 DOI=10.3389/fmicb.2022.949932 ISSN=1664-302X ABSTRACT=
Lactobacillaceae are Gram-positive rods, facultative anaerobes, and belong to the lactic acid bacteria (LAB) that frequently serve as probiotics. We systematically compared five LAB strains for the effects of different carbohydrates on their free-living and biofilm lifestyles. We found that fermentable sugars triggered an altered carrying capacity with strain specificity during planktonic growth. In addition, heterogeneous response to fermentable sugar was manifested in microbial aggregation (measured by imaging flow cytometry), colony development, and attachment to mucin. The acid production capacities of the strains were compatible and could not account for heterogeneity in their differential carrying capacity in liquid and on a solid medium. Among tested LAB strains,