AUTHOR=Alam Khorshed , Hao Jinfang , Zhong Lin , Fan Guoqing , Ouyang Qing , Islam Md. Mahmudul , Islam Saiful , Sun Hongluan , Zhang Youming , Li Ruijuan , Li Aiying 

TITLE=Complete genome sequencing and in silico genome mining reveal the promising metabolic potential in Streptomyces strain CS-7

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.939919

DOI=10.3389/fmicb.2022.939919

ISSN=1664-302X

ABSTRACT=<p>Gram-positive <italic>Streptomyces</italic> bacteria can produce valuable secondary metabolites. <italic>Streptomyces</italic> genomes include huge unknown silent natural product (NP) biosynthetic gene clusters (BGCs), making them a potential drug discovery repository. To collect antibiotic-producing bacteria from unexplored areas, we identified <italic>Streptomyces</italic> sp. CS-7 from mountain soil samples in Changsha, P.R. China, which showed strong antibacterial activity. Complete genome sequencing and prediction <italic>in silico</italic> revealed that its 8.4 Mbp genome contains a total of 36 BGCs for NPs. We purified two important antibiotics from this strain, which were structurally elucidated to be mayamycin and mayamycin B active against <italic>Staphylococcus aureus</italic>. We identified functionally a BGC for the biosynthesis of these two compounds by BGC direct cloning and heterologous expression in <italic>Streptomyces albus.</italic> The data here supported this <italic>Streptomyces</italic> species, especially from unexplored habitats, having a high potential for new NPs.</p>