AUTHOR=Wu Baiyila , Hu Zongfu , Wei Manlin , Yong Mei , Niu Huaxin 

TITLE=Effects of inoculation of Lactiplantibacillus plantarum and Lentilactobacillus buchneri on fermentation quality, aerobic stability, and microbial community dynamics of wilted Leymus chinensis silage

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.928731

DOI=10.3389/fmicb.2022.928731

ISSN=1664-302X

ABSTRACT=<p><italic>Leymus chinensis</italic> is an important crop that can be fed to ruminants. The purpose of this study was to investigate the roles of <italic>Lactiplantibacillus plantarum</italic> and <italic>Lentilactobacillus buchneri</italic> in fermentation quality, aerobic stability, and dynamics of wilted <italic>L. chinensis</italic> silage microorganisms. Wilted <italic>L. chinensis</italic> silages were ensiled with/without <italic>L. plantarum</italic> and <italic>L. buchneri</italic>. After 14 and 56 days of ensiling, the silos were opened and subjected to a 7-day aerobic deterioration test. This study looked at the composition of fermentation products as well as the microbial communities in silage. Silage inoculated with <italic>L. plantarum</italic> and <italic>L. buchneri</italic> had an increased lactic acid content as well as lactic acid bacterial (LAB) quantity, but a decrease in pH and levels of butyric acid, 2,3-butanediol, and ethanol was observed during ensiling. Non-treated and <italic>L. plantarum</italic>-treated silages deteriorated in the 7-day spoilage test after opening day-14 silos, whereas <italic>L. buchneri</italic>-inoculated silage showed no signs of deterioration. <italic>Lactobacillus</italic> abundance increased in the 7-day spoilage test after opening day-56 silos, while undesirable microorganisms such as <italic>Acetobacter</italic>, <italic>Bacillus</italic>, and molds, namely, <italic>Aspergillus</italic> and <italic>Penicillium</italic> were inhibited within <italic>L. plantarum</italic>- and <italic>L. buchneri</italic>-inoculated silages. The composition of fermentation products was related to the bacterial community, particularly <italic>Lactobacillus</italic>, <italic>Enterococcus</italic>, and <italic>Acetobacter</italic>. To summarize, <italic>L. plantarum</italic>- and <italic>L. buchneri</italic>-inoculated silage enhanced fermentation quality during ensiling and inhibited aerobic spoilage in a 7-day spoilage test of 56 days ensiling within wilted <italic>L. chinensis</italic> silage.</p>