AUTHOR=Jiao Jun , Qi Yong , He Peisheng , Wan Weiqiang , OuYang Xuan , Yu Yonghui , Wen Bohai , Xiong Xiaolu 

TITLE=Development of a Lateral Flow Strip-Based Recombinase-Aided Amplification for Active Chlamydia psittaci Infection

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.928025

DOI=10.3389/fmicb.2022.928025

ISSN=1664-302X

ABSTRACT=<p><italic>Chlamydia psittaci</italic> is the causative agent of psittacosis, a worldwide zoonotic disease. A rapid, specific, and sensitive diagnostic assay would be benefit for <italic>C. psittaci</italic> infection control. In this study, an assay combining recombinase-aided amplification and a lateral flow strip (RAA-LF) for the detection of active <italic>C. psittaci</italic> infection was developed. The RAA-LF assay targeted the <italic>CPSIT_RS02830</italic> gene of <italic>C. psittaci</italic> and could be accomplished in 15 min at a single temperature (39°C). The analytical sensitivity of the assay was as low as 1 × 10<sup>0</sup> copies/μl and no cross-reaction with some other intracellular pathogens was observed. Moreover, all feces samples from mice infected with <italic>C. psittaci</italic> at day-1 post-infection were positive in the RAA-LF assay. In conclusion, the RAA-LF assay provides a convenient, rapid, specific and sensitive method for detection of active <italic>C. psittaci</italic> infection and it is also suitable for <italic>C. psittaci</italic> detection in field.</p>