AUTHOR=Wang Xiaoran , Tang Jilang , Zhang Shixia , Zhang Nuannuan 

TITLE=Effects of Lactiplantibacillus plantarum 19-2 on immunomodulatory function and gut microbiota in mice

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.926756

DOI=10.3389/fmicb.2022.926756

ISSN=1664-302X

ABSTRACT=<p>This study aims to evaluate the effects of <italic>Lactiplantibacillus plantarum</italic> 19-2 (<italic>L. plantarum</italic> 19-2) on mice treated with the alkylating agent cyclophosphamide (CTX). Our findings show that <italic>L. plantarum</italic> 19-2 restored the spleen and thymus index and the number of white blood cells and lymphocytes% in CTX treated mice. Serum immunoglobulin levels in CTX-treated mice were increased by <italic>L. plantarum</italic> 19-2. In addition, as compared to the model group, <italic>L. plantarum</italic> 19-2 upregulated the content of SIgA, while <italic>L. plantarum</italic> 19-2 regulates the mRNA and protein expression levels of GATA-3, T-bet, IFN-γ, and IL-4 in small intestinal tissues, which adjusted mucosal barriers, structural status, and the balance of Helper T-cell 1 and Helper T-cell 2. <italic>Lactiplantibacillus plantarum</italic> 19-2 regulated the distribution of intestinal flora in mice, promoting the growth of <italic>Bacteroides</italic> and <italic>Proteobacteria</italic>. In addition, <italic>L. plantarum</italic> 19-2 inhibited the growth of several harmful bacteria, including <italic>Actinobacteria</italic> and <italic>Firmicutes</italic>.</p>