AUTHOR=Zheng Tianfei , Zhang Qianying , Wu Qiaoyin , Li Dongliang , Wu Xinying , Li Pinhe , Zhou Quanwei , Cai Wen , Zhang Juan , Du Guocheng 

TITLE=Effects of Inoculation With Acinetobacter on Fermentation of Cigar Tobacco Leaves

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.911791

DOI=10.3389/fmicb.2022.911791

ISSN=1664-302X

ABSTRACT=<p>Metabolic activity of the microbial community greatly affects the quality of cigar tobacco leaves (CTLs). To improve the quality of CTLs, two extrinsic microbes (<italic>Acinetobacter</italic> sp. 1H8 and <italic>Acinetobacter indicus</italic> 3B2) were inoculated into CTLs. The quality of CTLs were significantly improved after fermentation. The content of solanone, 6-methyl-5-hepten-2-one, benzeneacetic acid, ethyl ester, cyclohexanone, octanal, acetophenone, and 3,5,5-trimethyl-2-cyclohexen-1-one were significantly increased after inoculated <italic>Acinetobacter</italic> sp. 1H8. The inoculation of <italic>Acinetobacter</italic> sp. 1H8 enhanced the normal evolutionary trend of bacterial community. The content of trimethyl-pyrazine, 2,6-dimethyl-pyrazine, and megastigmatrienone were significantly increased after inoculated <italic>Acinetobacter indicus</italic> 3B2. The inoculation of <italic>Acinetobacter indicus</italic> 3B2 completely changed the original bacterial community. Network analysis revealed that <italic>Acinetobacter</italic> was negatively correlated with <italic>Aquabacterium</italic>, positively correlated with <italic>Bacillus</italic>, and had significant correlations with many volatile flavor compounds. This work may be helpful for improving fermentation product quality by regulating microbial community, and gain insight into the microbial ecosystem.</p>