AUTHOR=Arnaud Alexis P. , Cousin Ianis , Schmitt Françoise , Petit Thierry , Parmentier Benoit , Levard Guillaume , Podevin Guillaume , Guinot Audrey , DeNapoli Stéphan , Hervieux Erik , Flaum Valérie , De Vries Philine , Randuineau Gwénaëlle , David-Le Gall Sandrine , Buffet-Bataillon Sylvie , Boudry Gaëlle 

TITLE=Different Fecal Microbiota in Hirschsprung's Patients With and Without Associated Enterocolitis

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.904758

DOI=10.3389/fmicb.2022.904758

ISSN=1664-302X

ABSTRACT=<sec><title>Background and Objectives</title><p>Patients with Hirschsprung's disease are at risk of developing Hirschsprung-associated enterocolitis, especially in the first 2 years of life. The pathophysiology of this inflammatory disease remains unclear, and intestinal dysbiosis has been proposed in the last decade. The primary objective of this study was to evaluate in a large cohort if Hirschsprung-associated enterocolitis was associated with alterations of fecal bacterial composition compared with HD without enterocolitis in different age groups.</p></sec><sec><title>Methods</title><p>We analyzed the fecal microbiota structure of 103 Hirschsprung patients from 3 months to 16 years of age, all of whom had completed definitive surgery for rectosigmoid Hirschsprung. 16S rRNA gene sequencing allowed us to compare the microbiota composition between Hirschsprung's disease patients with (HAEC group) or without enterocolitis (HD group) in different age groups (0–2, 2–6, 6–12, and 12–16 years).</p></sec><sec><title>Results</title><p>Richness and diversity increased with age group but did not differ between HD and HAEC patients, irrespective of the age group. Relative abundance of Actinobacteria was lower in HAEC than in HD patients under 2 years of age (−66%, <italic>P</italic> = 0.045). Multivariate analysis by linear models (MaAsLin) considering sex, medications, birth mode, breast-feeding, and the Bristol stool scale, as well as surgery parameters, highlighted <italic>Flavonifractor plautii</italic> and <italic>Eggerthella lenta</italic>, as well as <italic>Ruminococcus gnavus</italic> group, as positively associated with Hirschsprung-associated enterocolitis in the 0–2 years age group.</p></sec><sec><title>Conclusion</title><p>Hirschsprung-associated enterocolitis was associated with features of intestinal dysbiosis in infants (0–2 years) but not in older patients. This could explain the highest rate of enterocolitis in this age group.</p></sec><sec><title>Clinical Trial Registration</title><p><ext-link ext-link-type="uri" xlink:href="https://clinicaltrials.gov/ct2/show/NCT02857205" xmlns:xlink="http://www.w3.org/1999/xlink">https://clinicaltrials.gov/ct2/show/NCT02857205</ext-link>, MICROPRUNG, NCT02857205, 02/08/2016.</p></sec>