AUTHOR=Tang Jianli , Chen Jianming , Liu Yang , Hu Jinjuan , Xia Ziyuan , Li Xiaomin , He Haocheng , Rang Jie , Sun Yunjun , Yu Ziquan , Cui Jun , Xia Liqiu 

TITLE=The Global Regulator PhoU Positively Controls Growth and Butenyl-Spinosyn Biosynthesis in Saccharopolyspora pogona

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.904627

DOI=10.3389/fmicb.2022.904627

ISSN=1664-302X

ABSTRACT=<p>Butenyl-spinosyn, a highly effective biological insecticide, is produced by <italic>Saccharopolyspora pogona</italic>. However, its application has been severely hampered by its low yield. Recent studies have shown that PhoU plays a pivotal role in regulating cell growth, secondary metabolite biosynthesis and intracellular phosphate levels. Nevertheless, the function of PhoU remains ambiguous in <italic>S. pogona</italic>. In this study, we investigated the effects of PhoU on the growth and the butenyl-spinosyn biosynthesis of <italic>S. pogona</italic> by constructing the mutants. Overexpression of <italic>phoU</italic> increased the production of butenyl-spinosyn to 2.2-fold that of the wild-type strain. However, the <italic>phoU</italic> deletion resulted in a severe imbalance of intracellular phosphate levels, and suppression of the growth and butenyl-spinosyn biosynthesis. Quantitative Real-time PCR (qRT-PCR) analysis, distinctive protein detection and mass spectrometry revealed that PhoU widely regulated primary metabolism, energy metabolism and DNA repair, which implied that PhoU influences the growth and butenyl-spinosyn biosynthesis of <italic>S. pogona</italic> as a global regulator.</p>