AUTHOR=Zuo Kangjia , Li Huanan , Chen Jianhui , Ran Qiuping , Huang Mengtian , Cui Xinxin , He Lili , Liu Jiashu , Jiang Zhengbing 

TITLE=Effective Biotransformation of Variety of Guaiacyl Lignin Monomers Into Vanillin by Bacillus pumilus

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.901690

DOI=10.3389/fmicb.2022.901690

ISSN=1664-302X

ABSTRACT=<p>Biotransformation has gained increasing attention due to its being an eco-friendly way for the production of value-added chemicals. The present study aimed to assess the potential of <italic>Bacillus pumilus</italic> ZB1 on guaiacyl lignin monomers biotransformation for the production of vanillin. Consequently, isoeugenol, eugenol, and vanillyl alcohol could be transformed into vanillin by <italic>B. pumilus</italic> ZB1. Based on the structural alteration of masson pine and the increase of total phenol content in the supernatant, <italic>B. pumilus</italic> ZB1 exhibited potential in lignin depolymerization and valorization using masson pine as the substrate. As the precursors of vanillin, 61.1% of isoeugenol and eugenol in pyrolyzed bio-oil derived from masson pine could be transformed into vanillin by <italic>B. pumilus</italic> ZB1. Four monooxygenases with high specific activity were identified that were involved in the transformation process. Thus, <italic>B. pumilus</italic> ZB1 could emerge as a candidate in the biosynthesis of vanillin by using wide guaiacyl precursors as the substrates.</p>