AUTHOR=Wang Guisheng , Yin Zhenhua , Wang Senye , Yuan Yilin , Chen Yan , Kang Wenyi 

TITLE=Diversified Polyketides With Anti-inflammatory Activities From Mangrove Endophytic Fungus Daldinia eschscholtzii KBJYZ-1

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.900227

DOI=10.3389/fmicb.2022.900227

ISSN=1664-302X

ABSTRACT=<p>In total, five new polyketide derivatives: eschscholin B (<bold>2</bold>), dalditone A and B (<bold>3</bold> and <bold>4</bold>), (1<italic>R</italic>, 4<italic>R</italic>)-5-methoxy-1,2,3,4-tetrahydronaphthalene-1,4-dio (<bold>5</bold>), and daldilene A (<bold>6</bold>), together with 10 known as analogs (<bold>1</bold>, <bold>7</bold>–<bold>15</bold>) were isolated from the mangrove endophytic fungus <italic>Daldinia eschscholtzii</italic> KBJYZ-1. Their structures and absolute configurations were established by extensive analysis of NMR and HRESIMS spectra data combined with ECD calculations and the reported literature. Compounds <bold>2</bold> and <bold>6</bold> showed significant cell-based anti-inflammatory activities with IC<sub>50</sub> values of 19.3 and 12.9 μM, respectively. In addition, western blot results suggested that compound <bold>2</bold> effectively inhibits the expression of iNOS and COX-2 in LPS-induced RAW264.7 cells. Further molecular biology work revealed the potential mechanism of <bold>2</bold> exerts anti-inflammatory function by inactivating the MAPK and NF–κB signaling pathways.</p>