AUTHOR=Li Yuan , Qian Yilin , Lou Xiaowei , Hu Zhiheng , Hu Yaqin , Zeng Mingyong , Liu Zunying 

TITLE=LuxS in Lactobacillus plantarum SS-128 Improves the Texture of Refrigerated Litopenaeus vannamei: Mechanism Exploration Using a Proteomics Approach

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.892788

DOI=10.3389/fmicb.2022.892788

ISSN=1664-302X

ABSTRACT=<p>This study illustrated the texture changes of <italic>Shewanella baltica</italic>-inoculated <italic>Litopenaeus vannamei</italic> during refrigerated storage with the exogenous addition of <italic>Lactobacillus plantarum</italic> SS-128. The group inoculated with SS-128 had an improved texture compared with that inoculated with the <italic>luxS</italic>-mutant group (Δ<italic>luxS</italic>). Proteomics were conducted to analyze the protein alterations in <italic>L. vannamei</italic> and supernatant, respectively. During storage, many texture-related proteins, including myosin heavy chain and beta-actin, were maintained due to <italic>luxS</italic>. Some endogenous enzymes related to the energy metabolism and hydrolysis of <italic>L. vannamei</italic> were downregulated. The <italic>luxS</italic>-induced interaction with <italic>S. baltica</italic> showed significant changes in the expression of some critical enzymes and pathways. The ATP-dependent zinc metalloprotease FtsH and protease subunit HslV were downregulated, and the oxidative phosphorylation and glycosaminoglycan degradation pathways in <italic>S. baltica</italic> were inhibited, resulting in the slow deterioration of <italic>L. vannamei</italic>. By exploring the mechanism underlying SS-128-led manipulation of the metabolism of spoilage bacteria, we clarified the texture maintenance mechanism of <italic>luxS</italic> in SS-128, providing theoretical evidence for SS-128 application in food preservation.</p>