AUTHOR=Zhang Zixiong , Zhang Ning , Guo Saisai , Liu Qian , Wang Shujie , Zhang Ao , Yi Dongrong , Zhao Jianyuan , Li Quanjie , Wang Jing , Zhang Yongxin , Ma Ling , Ding Jiwei , Cen Shan , Li Xiaoyu TITLE=The Zinc-Finger protein ZCCHC3 inhibits LINE-1 retrotransposition JOURNAL=Frontiers in Microbiology VOLUME=13 YEAR=2022 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.891852 DOI=10.3389/fmicb.2022.891852 ISSN=1664-302X ABSTRACT=

Long-interspersed element 1 (LINE-1) is an autonomous non-LTR retrotransposon. Its replication can cause mutation and rearrangement of host genomic DNA, which may result in serious genetic diseases. Host cells therefore developed defense strategies to restrict LINE-1 mobilization. In this study, we reported that CCHC-type zinc-finger protein ZCCHC3 can repress LINE-1 retrotransposition, and this activity is closely related to its zinc-finger domain. Further studies show that ZCCHC3 can post-transcriptionally diminish the LINE-1 RNA level. The association of ZCCHC3 with both LINE-1 RNA and ORF1 suggests that ZCCHC3 interacts with LINE-1 RNP and consequently causes its RNA degradation. These data demonstrate collectively that ZCCHC3 contributes to the cellular control of LINE-1 replication.