AUTHOR=Zhao Teng , Li Bo-qi , Gao He-ting , Xing Dan , Li Man-jin , Dang Yun-qi , Zhang Heng-duan , Zhao Yue-e , Liu Zhu , Li Chun-xiao 

TITLE=Metagenome Sequencing Reveals the Microbiome of Aedes albopictus and Its Possible Relationship With Dengue Virus Susceptibility

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.891151

DOI=10.3389/fmicb.2022.891151

ISSN=1664-302X

ABSTRACT=<p>Dengue fever virus (DENV) is a mosquito-borne flavivirus that poses a serious risk to human health. <italic>Aedes albopictus</italic> is a widely distributed vector of dengue fever in China. Based on the impact of physiological activity, the microbiome in <italic>A. albopictus</italic> will provide a novel environment-friendly approach to control DENV transmission. We performed metagenomic sequencing on <italic>A. albopictus</italic> before and after exposure to DENV blood meal to detect microbiome variation of <italic>A. albopictus</italic> with different susceptibilities to DENV. The dominant phyla in <italic>A. albopictus</italic> microbiome were <italic>Proteobacteria</italic> and <italic>Ascomycota</italic>, and the dominant genera were <italic>Aspergillus</italic> and <italic>Metarhizium</italic>. <italic>Gammaproteobacteria bacterium</italic>, <italic>Lactobacillus harbinensis</italic>, and <italic>Neurospora crassa</italic> differed significantly after DENV infection. There were 15 different microorganisms found to be involved in mosquito immunity and metabolism, such as <italic>Alphaproteobacteria bacterium</italic>, <italic>Methyloglobulus morosus</italic>, and <italic>Shigella sonnei</italic>, which might have an impact on the DENV susceptibility of <italic>A. albopictus</italic>. It was hypothesized that the lack of specific bacteria may lead to increased susceptibility of <italic>A. albopictus</italic> to DENV. Interventions in the microbiome composition or specific bacteria of <italic>A. albopictus</italic> may affect the susceptibility to DENV and control the mosquito-borne diseases efficiently.</p>