AUTHOR=Giraud Carolane , Callac Nolwenn , Boulo Viviane , Lam Jean-Sébastien , Pham Dominique , Selmaoui-Folcher Nazha , Wabete Nelly 

TITLE=The Active Microbiota of the Eggs and the Nauplii of the Pacific Blue Shrimp Litopenaeus stylirostris Partially Shaped by a Potential Vertical Transmission

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.886752

DOI=10.3389/fmicb.2022.886752

ISSN=1664-302X

ABSTRACT=<p>The many ecological niches present in an organism harbor distinct microorganisms called microbiota. Different factors can influence the establishment of these commensal microbial communities. In a previous article, we have concluded that some bacterial lineages associated with the early larval stages of the Pacific blue shrimp <italic>Litopenaeus stylirostris</italic> could be acquired from the breeders <italic>via</italic> a potential vertical transmission. The present study was conducted in order to investigate this hypothesis. Using HiSeq sequencing of the V4 region of 16S rRNA gene, we analyzed the active microbiota associated with the eggs and the nauplii of <italic>L. stylirsotris</italic> as well as with the reproductive organs of their breeders. Microbial communities associated with the rearing water were also considered to discriminate environmental microbial lineages. Using these analyses, we highlight a set of core bacterial families present in all samples and composed of members of <italic>Colwelliaceae</italic>, <italic>Alteromonadaceae</italic>, <italic>Pseudoalteromonadaceae</italic>, <italic>Saccharospirillaceae</italic>, <italic>Oceanospirillaceae</italic>, <italic>Vibrionaceae</italic>, <italic>Burkholderiaceae</italic>, <italic>Rhodobacteraceae</italic>, <italic>Flavobacteraceae</italic>, and <italic>Corynebacteriaceae</italic>; showing the importance of the environment in the establishment of the larval microbiota. We also present specific bacteria affiliated to the <italic>Arcobacteraceae</italic>, <italic>Rhodobacteraceae</italic>, <italic>Comamonadaceae</italic>, and <italic>Colwelliaceae</italic> families, which were only found in the breeders and their offspring strengthening the hypothesis of a potential vertical transmission shaping the active microbiota of the eggs and the nauplii of <italic>L. stylirostris</italic>.</p>