AUTHOR=Xu Shiqiang , Zhang Xiangxiang , Xu Kai , Wang Zhanqi , Zhou Xueping , Jiang Lei , Jiang Tong 

TITLE=Strawberry Vein Banding Virus Movement Protein P1 Interacts With Light-Harvesting Complex II Type 1 Like of Fragaria vesca to Promote Viral Infection

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.884044

DOI=10.3389/fmicb.2022.884044

ISSN=1664-302X

ABSTRACT=<p>Chlorophyll a/b-binding protein of light-harvesting complex II type 1 like (LHC II-1L) is an essential component of photosynthesis, which mainly maintains the stability of the electron transport chain. However, how the LHC II-1L protein of <italic>Fragaria vesca</italic> (FvLHC II-1L) affects viral infection remains unclear. In this study, we demonstrated that the movement protein P1 of strawberry vein banding virus (SVBV P1) interacted with FvLHC II-1L <italic>in vivo</italic> and <italic>in vitro</italic> by bimolecular fluorescence complementation and pull-down assays. SVBV P1 was co-localized with FvLHC II-1L at the edge of epidermal cells of <italic>Nicotiana benthamiana</italic> leaves, and FvLHC II-1L protein expression was upregulated in SVBV-infected <italic>F. vesca</italic>. We also found that FvLHC II-1L effectively promoted SVBV P1 to compensate for the intercellular movement of movement-deficient potato virus X (PVX<sup>ΔP25</sup>) and the systemic movement of movement-deficient cucumber mosaic virus (CMV<sup>ΔMP</sup>). Transient overexpression of FvLHC II-1L and inoculation of an infectious clone of SVBV showed that the course of SVBV infection in <italic>F. vesca</italic> was accelerated. Collectively, the results showed that SVBV P1 protein can interact with FvLHC II-1L protein, which in turn promotes <italic>F. vesca</italic> infection by SVBV.</p>