AUTHOR=Liu Yifei , Wang Yawen , Chen Xinyue , Jin Junhua , Liu Hui , Hao Yanling , Zhang Hongxing , Xie Yuanhong 

TITLE=BasS/BasR Two-Component System Affects the Sensitivity of Escherichia coli to Plantaricin BM-1 by Regulating the Tricarboxylic Acid Cycle

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.874789

DOI=10.3389/fmicb.2022.874789

ISSN=1664-302X

ABSTRACT=<p>Plantaricin BM-1, a class IIa bacteriocin produced by <italic>Lactiplantibacillus plantarum</italic> BM-1, exhibits significant antibacterial activity against many gram-positive and gram-negative bacteria. However, the mechanism underlying the action of class IIa bacteriocins against gram-negative bacteria remains to be explored. This study aimed to investigate the role of the BasS/BasR two-component system (TCS) in <italic>Escherichia coli</italic> (<italic>E. coli</italic>) K12 response to plantaricin BM-1. The IC<sub>50</sub> values for plantaricin BM-1 in <italic>E. coli</italic> K12, <italic>basS</italic> mutant (<italic>E. coli</italic> JW4073), and <italic>basR</italic> mutant (<italic>E. coli</italic> JW4074) strains were found to be 10.85, 8.94, and 7.62 mg/mL, respectively. Growth curve experiments showed that mutations in the BasS/BasR TCS led to an increase in the sensitivity of <italic>E. coli</italic> K12 to plantaricin BM-1 and that after gene complementation, the complemented mutant strain regained its original sensitivity. Proteomic analysis showed that 100 and 26 proteins were upregulated and 62 and 58 proteins were downregulated in <italic>E. coli</italic> JW4073 and <italic>E. coli</italic> JW4074, respectively. These differential proteins, which exhibited different molecular functions and participated in different molecular pathways, were mainly concentrated in the cytoplasm. More specifically, mutations in <italic>basS</italic> and <italic>basR</italic> were found to affect the synthesis and metabolism of many substances in <italic>E. coli</italic>, including many important amino acids and enzymes involved in cellular activities. In addition, 14 proteins, including 8 proteins involved in the tricarboxylic acid (TCA) cycle, were found to be downregulated in both <italic>E. coli</italic> JW4073 and <italic>E. coli</italic> JW4074. Growth curve experiments showed that the deletion of these proteins could increase the sensitivity of <italic>E. coli</italic> to plantaricin BM-1. Therefore, we speculate that TCA pathway regulation may be an important mechanism by which the BasS/BasR TCS regulates the sensitivity of <italic>E. coli</italic> to plantaricin BM-1. This finding will facilitate the determination of the mechanism underlying the action of class IIa bacteriocins against gram-negative bacteria.</p>