AUTHOR=Haase Alexander , Sawers R. Gary 

TITLE=Exchange of a Single Amino Acid Residue in the HybG Chaperone Allows Maturation of All H2-Activating [NiFe]-Hydrogenases in Escherichia coli

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.872581

DOI=10.3389/fmicb.2022.872581

ISSN=1664-302X

ABSTRACT=<p>The biosynthesis of the NiFe(CN)<sub>2</sub>CO organometallic cofactor of [NiFe]-hydrogenase (Hyd) involves several discreet steps, including the synthesis of the Fe(CN)<sub>2</sub>CO group on a HypD-HypC scaffold complex. HypC has an additional function in transferring the Fe(CN)<sub>2</sub>CO group to the apo-precursor of the Hyd catalytic subunit. Bacteria that synthesize more than one Hyd enzyme often have additional HypC-type chaperones specific for each precursor. The specificity determinants of this large chaperone family are not understood. <italic>Escherichia coli</italic> synthesizes two HypC paralogs, HypC and HybG. HypC delivers the Fe(CN)<sub>2</sub>CO group to pre-HycE, the precursor of the H<sub>2</sub>-evolving Hyd-3 enzyme, while HybG transfers the group to the pre-HybC of the H<sub>2</sub>-oxidizing Hyd-2 enzyme. We could show that a conserved histidine residue around the amino acid position 50 in both HypC and HybG, when exchanged for an alanine, resulted in a severe reduction in the activity of its cognate Hyd enzyme. This reduction in enzyme activity proved to be due to the impaired ability of the chaperones to interact with HypD. Surprisingly, and only in the case of the HybG<sub><italic>H</italic>52<italic>A</italic></sub> variant, its co-synthesis with HypD improved its interaction with pre-HycE, resulting in the maturation of Hyd-3. This study demonstrates that the conserved histidine residue helps enhance the interaction of the chaperone with HypD, but additionally, and in <italic>E. coli</italic> only for HybG, acts as a determinant to prevent the inadvertent maturation of the wrong large-subunit precursor. This study identifies a new level of control exerted by a bacterium synthesizing multiple [NiFe]-Hyd to ensure the correct enzyme is matured only under the appropriate physiological conditions.</p>