AUTHOR=Li Liangliang , Sun Weiyao , Hu Qifan , Wang Tongtong , Zhu Guang , Zhao Qin , Zhou En-Min 

TITLE=Identification of MYH9 Key Domain Involved in the Entry of PRRSV Into Permissive Cells

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.865343

DOI=10.3389/fmicb.2022.865343

ISSN=1664-302X

ABSTRACT=Porcine reproductive and respiratory syndrome virus (PRRSV) is an important pathogen that causes huge losses economically to the pig industry worldwide. Previous research suggested that receptor-dependence is necessary for PRRSV infection. MYH9 and CD163 are indispensable for PRRSV entry into a porcine alveolar macrophage. In the present study, human MYH9 (hMYH9) and mouse MYH9 (mMYH9), similar to swine MYH9, could also accelerate PRRSV infection in pCD163-mediated cell lines. Knockdown of MYH9 activity using the specific small interfering RNA or inhibitor (Blebbistatin) concomitantly decreased PRRSV infection. C-terminal fragment of MYH9 (PRA) proteins from different mammalian species contains a conserved binding domain (aa1676-1791) for PRRSV binding since the recombinant MYH91676-1791protein could inhibit the PRRSV infection significantly. Furthermore, the specific polyclonal antibody of MYH91676-1791 could block PRRSV infection in host cells. These data strongly supported that MYH9, a very important cofactor, participated in PRRSV entry into target cells, which may develop a new therapeutic agent to control PRRSV infection.