AUTHOR=Wang Dangfeng , Cui Fangchao , Ren Likun , Tan Xiqian , Lv Xinran , Li Qiuying , Li Jianrong , Li Tingting 

TITLE=Complete Genome Analysis Reveals the Quorum Sensing-Related Spoilage Potential of Pseudomonas fluorescens PF08, a Specific Spoilage Organism of Turbot (Scophthalmus maximus)

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.856802

DOI=10.3389/fmicb.2022.856802

ISSN=1664-302X

ABSTRACT=<p><italic>Pseudomonas fluorescens</italic> is a common specific spoilage organism (SSO) of aquatic products. The spoilage ability of SSO can be regulated by the quorum sensing (QS) system. However, the QS system in <italic>P. fluorescens</italic> and their relationship with the spoilage potential have not been systematically analyzed. In the present study, the complete genome of <italic>P. fluorescens</italic> PF08 isolated from spoilage turbot was sequenced. The identification of key genes that involved in the QS, enzyme synthesis, sulfur, and amino acid metabolism explained the spoilage potential of <italic>P. fluorescens</italic> PF08. Results of quantitative real-time PCR revealed the key role of the <italic>P. fluorescens</italic> PF08 QS system in regulating the transcription of spoilage-related genes and its sensitivity to environmental stress. These findings provide insight into the spoilage features of <italic>P. fluorescens</italic> PF08 from a genomic perspective. The knowledge may be valuable in the development of new strategies for the targeted inhibition of aquatic product spoilage based on QS interference.</p>