AUTHOR=Smith Paul E. , Kelly Alan K. , Kenny David A. , Waters Sinéad M. 

TITLE=Differences in the Composition of the Rumen Microbiota of Finishing Beef Cattle Divergently Ranked for Residual Methane Emissions

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.855565

DOI=10.3389/fmicb.2022.855565

ISSN=1664-302X

ABSTRACT=<p>With the advent of high throughput technology, it is now feasible to study the complex relationship of the rumen microbiota with methanogenesis in large populations of ruminant livestock divergently ranked for enteric emissions. Recently, the residual methane emissions (RME) concept has been identified as the optimal phenotype for assessing the methanogenic potential of ruminant livestock due to the trait’s independence from animal productivity but strong correlation with daily methane emissions. However, there is currently a dearth of data available on the bacterial and archaeal microbial communities residing in the rumens of animals divergently ranked for RME. Therefore, the objective of this study was to investigate the relationship between the rumen microbiota and RME in a population of finishing beef cattle. Methane emissions were estimated from individual animals using the GreenFeed Emissions Monitoring system for 21 days over a mean feed intake measurement period of 91 days. Residual methane emissions were calculated for 282 crossbred finishing beef cattle, following which a ∼30% difference in all expressions of methane emissions was observed between high and low RME ranked animals. Rumen fluid samples were successfully obtained from 268 animals during the final week of the methane measurement period using a trans-oesophageal sampling device. Rumen microbial DNA was extracted and subjected to 16S rRNA amplicon sequencing. Animals ranked as low RME had the highest relative abundances (<italic>P</italic> &lt; 0.05) of lactic-acid-producing bacteria (<italic>Intestinibaculum</italic>, <italic>Sharpea</italic>, and <italic>Olsenella</italic>) and <italic>Selenomonas</italic>, and the lowest (<italic>P</italic> &lt; 0.05) proportions of <italic>Pseudobutyrivibrio</italic>, <italic>Butyrivibrio</italic>, and <italic>Mogibacterium</italic>. Within the rumen methanogen community, an increased abundance (<italic>P</italic> &lt; 0.05) of the genus <italic>Methanosphaera</italic> and <italic>Methanobrevibacter</italic> RO clade was observed in low RME animals. The relative abundances of both <italic>Intestinibaculum</italic> and <italic>Olsenella</italic> were negatively correlated (<italic>P</italic> &lt; 0.05) with RME and positively correlated with ruminal propionate. A similar relationship was observed for the abundance of <italic>Methanosphaera</italic> and the <italic>Methanobrevibacter</italic> RO clade. Findings from this study highlight the ruminal abundance of bacterial genera associated with the synthesis of propionate <italic>via</italic> the acrylate pathway, as well as the methanogens <italic>Methanosphaera</italic> and members of the <italic>Methanobrevibacter</italic> RO clade as potential microbial biomarkers of the methanogenic potential of beef cattle.</p>