AUTHOR=Wang Tingting , Sun Silu , Xu Yanxing , Waigi Michael Gatheru , Odinga Emmanuel Stephen , Vasilyeva Galina K. , Gao Yanzheng , Hu Xiaojie 

TITLE=Nitrogen Regulates the Distribution of Antibiotic Resistance Genes in the Soil–Vegetable System

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.848750

DOI=10.3389/fmicb.2022.848750

ISSN=1664-302X

ABSTRACT=<p>The increasing antibiotic resistance genes (ARGs) in fertilizer-amended soils can potentially enter food chains through their transfer in a soil–vegetable system, thus, posing threats to human health. As nitrogen is an essential nutrient in agricultural production, the effect of nitrogen (in the forms NH<sub>4</sub><sup>+</sup>-N and NO<sub>3</sub><sup>−</sup>-N) on the distribution of ARGs (<italic>blaTEM-1</italic>, <italic>sul1, cmlA, str</italic>, and <italic>tetO</italic>) and a mobile genetic element (MGE; <italic>tnpA-4</italic>) in a soil–Chinese cabbage system was investigated. Not all the tested genes could transfer from soil to vegetable. For transferable ones (<italic>blaTEM-1</italic>, <italic>sul1</italic>, and <italic>tnpA-4</italic>), nitrogen application influenced their abundances in soil and vegetable but did not impact their distribution patterns (i.e., preference to either leaf or root tissues). For ARGs in soil, effects of nitrogen on their abundances varied over time, and the positive effect of NH<sub>4</sub><sup>+</sup>-N was more significant than that of NO<sub>3</sub><sup>−</sup>-N. The ARG accumulation to vegetables was affected by nitrogen application, and the nitrogen form was no longer a key influencing factor. In most cases, ARGs were found to prefer being enriched in roots, and nitrogen application may slightly affect their migration from root to leaf. The calculated estimated human intake values indicated that both children and adults could intake 10<sup>6</sup>–10<sup>7</sup> copies of ARGs per day from Chinese cabbage consumption, and nitrogen application affected ARG intake to varying degrees. These results provided a new understanding of ARG distribution in vegetables under the agronomic measures such as nitrogen application, which may offer knowledge for healthy vegetable cultivation in future.</p>