AUTHOR=Luo Xinhua , Mu Kai , Zhao Yujie , Zhang Jin , Qu Ying , Hu Dakang , Jia Yifan , Dai Piaopiao , Weng Jian , Wang Dongguo , Yu Lianhua 

TITLE=Emergence of blaNDM– 1-Carrying Aeromonas caviae K433 Isolated From Patient With Community-Acquired Pneumonia

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.825389

DOI=10.3389/fmicb.2022.825389

ISSN=1664-302X

ABSTRACT=<p>To demonstrate the detailed genetic characteristics of a <italic>bla</italic><sub>NDM–1</sub>-carrying multidrug-resistant <italic>Aeromonas caviae</italic> strain, the complete genome of the <italic>A. caviae</italic> strain K433 was sequenced by Illumina HiSeq and Oxford nanopore platforms, and mobile genetic elements associated with antibiotic resistance genes were analyzed by a series of bioinformatics methods. <italic>A. caviae</italic> K433 which was determined to produce class B carbapenemase, was resistant to most antibiotics tested except amikacin. The genome of K433 consisted of a chromosome cK433 (6,482-kb length) and two plasmids: pK433-qnrS (7.212-kb length) and pK433-NDM (200.855-kb length), the last being the first investigated <italic>bla</italic><sub>NDM</sub>-carrying plasmid from <italic>Aeromonas</italic> spp. By comparison of the backbone and MDR regions from the plasmids studied, they involved a highly homologous sequence structure. This study provides in-depth genetic insights into the plasmids integrated with <italic>bla</italic><sub>NDM</sub>-carrying genetic elements from <italic>Aeromonas</italic> spp.</p>