AUTHOR=Wu Yao-Dong , Dai Guo-Dong , Li Li , Littlewood D. Timothy J. , Ohiolei John Asekhaen , Zhang Lin-Sheng , Guo Ai-Min , Wu Yan-Tao , Ni Xing-Wei , Shumuye Nigus Abebe , Li Wen-Hui , Zhang Nian-Zhang , Fu Bao-Quan , Fu Yong , Yan Hong-Bin , Jia Wan-Zhong 

TITLE=Expansion of Cyclophyllidea Biodiversity in Rodents of Qinghai-Tibet Plateau and the “Out of Qinghai-Tibet Plateau” Hypothesis of Cyclophyllideans

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.747484

DOI=10.3389/fmicb.2022.747484

ISSN=1664-302X

ABSTRACT=<p>The Cyclophyllidea comprises the most species-rich order of tapeworms (Platyhelminthes, Cestoda) and includes species with some of the most severe health impact on wildlife, livestock, and humans. We collected seven Cyclophyllidea specimens from rodents in Qinghai-Tibet Plateau (QTP) and its surrounding mountain systems, of which four specimens in QTP were unsequenced, representing “putative new species.” Their complete mitochondrial (<italic>mt</italic>) genomes were sequenced and annotated. Phylogenetic reconstruction of partial 28S rDNA, <italic>cox</italic>1 and <italic>nad</italic>1 datasets provided high bootstrap frequency support for the categorization of three “putative new species,” assigning each, respectively, to the genera <italic>Mesocestoides</italic>, <italic>Paranoplocephala</italic>, and <italic>Mosgovoyia</italic>, and revealing that some species and families in these three datasets, which contain 291 species from nine families, may require taxonomic revision. The partial 18S rDNA phylogeny of 29 species from Taeniidae provided high bootstrap frequency support for the categorization of the “putative new species” in the genus <italic>Hydatigera</italic>. Combined with the current investigation, the other three known Taeniidae species found in this study were <italic>Taenia caixuepengi</italic>, <italic>T. crassiceps</italic>, and <italic>Versteria mustelae</italic> and may be widely distributed in western China. Estimates of divergence time based on <italic>cox</italic>1 + <italic>nad</italic>1 fragment and <italic>mt</italic> protein-coding genes (PCGs) showed that the differentiation rate of Cyclophyllidea species was strongly associated with the rate of change in the biogeographic scenarios, likely caused by the uplift of the QTP; i.e., species differentiation of Cyclophyllidea might be driven by host-parasite co-evolution caused by the uplift of QTP. We propose an “out of QTP” hypothesis for the radiation of these cyclophyllidean tapeworms.</p>