AUTHOR=Xu Jing , Wang Bo , Wang Ming-Qing , Gao Jian-Jie , Li Zhen-Jun , Tian Yong-Sheng , Peng Ri-He , Yao Quan-Hong 

TITLE=Metabolic Engineering of Escherichia coli for Methyl Parathion Degradation

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.679126

DOI=10.3389/fmicb.2022.679126

ISSN=1664-302X

ABSTRACT=<p>Organophosphate compounds are widely used in pesticides to control weeds, crop diseases, and insect pests. Unfortunately, these synthetic compounds are hazardous and toxic to all types of living organisms. In the present work, <italic>Escherichia coli</italic> was bioengineered to achieve methyl parathion (MP) degradation via the introduction of six synthetic genes, namely, <italic>opdS</italic>, <italic>pnpAS</italic>, <italic>pnpBS</italic>, <italic>pnpCS</italic>, <italic>pnpDS</italic>, and <italic>pnpES</italic>, to obtain a new transformant, BL-MP. MP and its subsequent decomposition intermediates were completely degraded by this transformant to enter the metabolites of multiple anabolic pathways. The MP-degraded strain created in this study may be a promising candidate for the bioremediation of MP and potential toxic intermediates.</p>