AUTHOR=Srimahaeak Thanyaporn , Thongdee Narumon , Chittrakanwong Jurairat , Atichartpongkul Sopapan , Jaroensuk Juthamas , Phatinuwat Kamonwan , Phaonakrop Narumon , Jaresitthikunchai Janthima , Roytrakul Sittiruk , Mongkolsuk Skorn , Fuangthong Mayuree 

TITLE=Pseudomonas aeruginosa GidA modulates the expression of catalases at the posttranscriptional level and plays a role in virulence

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1079710

DOI=10.3389/fmicb.2022.1079710

ISSN=1664-302X

ABSTRACT=<p><italic>Pseudomonas aeruginosa gidA</italic>, which encodes a putative tRNA-modifying enzyme, is associated with a variety of virulence phenotypes. Here, we demonstrated that <italic>P. aeruginosa gidA</italic> is responsible for the modifications of uridine in tRNAs <italic>in vivo</italic>. Loss of <italic>gidA</italic> was found to have no impact on the mRNA levels of <italic>katA</italic> and <italic>katB</italic>, but it decreased KatA and KatB protein levels, resulting in decreased total catalase activity and a hydrogen peroxide-sensitive phenotype. Furthermore, <italic>gidA</italic> was found to affect flagella-mediated motility and biofilm formation; and it was required for the full virulence of <italic>P. aeruginosa</italic> in both <italic>Caenorhabditis elegans</italic> and macrophage models. Together, these observations reveal the posttranscriptional impact of <italic>gidA</italic> on the oxidative stress response, highlight the complexity of catalase gene expression regulation, and further support the involvement of <italic>gidA</italic> in the virulence of <italic>P. aeruginosa</italic>.</p>