AUTHOR=Yao Qing , Xie Tingting , Fu Yu , Wan Jiajia , Zhang Wendie , Gao Xuejun , Huang Jing , Sun Diangang , Zhang Fuxian , Bei Weicheng , Lei Liancheng , Liu Feng 

TITLE=The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1079390

DOI=10.3389/fmicb.2022.1079390

ISSN=1664-302X

ABSTRACT=<sec><title>Introduction</title><p>To survive in various hostile environments, two-component system is an adaptive mechanism for diverse bacteria. Activity of the CpxA/CpxR two-component system contributes to coping with different stimuli, such as pH, osmotic and heat stress.</p></sec><sec><title>Methods</title><p>However, the role of the CpxA/CpxR system in cold resistance is little-known. In this study, we showed that CpxA/CpxRwas critical for <italic>A. pleuropneumoniae</italic> growth under cold stress.</p></sec><sec><title>Results</title><p><italic>β</italic>-Galactosidaseanalysis showed that CpxA/CpxR positively regulated the predicted cold stress gene <italic>cspC</italic>. The mutant for cold stress gene <italic>cspC</italic> was impaired in the optimal growth of <italic>A. pleuropneumoniae</italic> under cold stress. Furthermore, electrophoretic mobility shift assays demonstrated that CpxR-P could directly regulate the transcription of the cold stress gene <italic>cspC</italic>.</p></sec><sec><title>Discussion</title><p>These results presented in this study illustrated that the CpxA/CpxR system plays an important role in cold resistance by upregulating expression of <italic>CspC</italic>. The data give new insights into how <italic>A. pleuropneumoniae</italic> survives in cold stress.</p></sec>