AUTHOR=Zhu Fangfang , Zhao Bei , Min Wenwen , Li Jiang 

TITLE=Characteristics of groundwater microbial communities and the correlation with the environmental factors in a decommissioned acid in-situ uranium mine

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1078393

DOI=10.3389/fmicb.2022.1078393

ISSN=1664-302X

ABSTRACT=<p>Microorganisms play an important role in the bioremediation process for the decommissioned acid <italic>in-situ</italic> leaching uranium mine. It is crucial to understand the original microbial community characteristics before the <italic>in-situ</italic> bioremediation. However, there are limited studies on the groundwater microbial characteristics in the decommissioned acid <italic>in-situ</italic> uranium mine. To this end, we collected groundwater samples, including the groundwater that originally residual in the borehole (RW) and the aquifer water (AW), from a decommissioned acid <italic>in-situ</italic> uranium mine in the southern margin of Ili Basin in Xinjiang, China. The occurrence characteristics of the groundwater microbial communities and their correlation with environmental factors were systematically studied based on the high throughput 16S rRNA gene sequencing data and geochemical data. Results found that the AW samples had higher alpha- and beta- diversity than the RW samples. The relative abundance of <italic>Sporosarcina</italic>, <italic>Sulfobacillus</italic>, <italic>Pedobacter</italic> and <italic>Pseudomonas</italic> were significantly different in the AW and RW samples, which had significant correlation with pH, metals, and sulfate, etc. A series of reducing microorganisms were discovered, such as sulfate reduction (e.g., <italic>Desulfosporosinus</italic>) and metal reduction (e.g., <italic>Arthrobacter</italic>, <italic>Bacillus</italic>, <italic>Clostridium</italic>, <italic>Pseudomonas</italic>, and <italic>Rhodanobacter</italic>), which have the potential to attenuate sulfate and uranium in groundwater. In addition, we found that pH and redox potential (Eh) were the dominant environmental factors affecting the microbial composition. This study extends our knowledge of microbial community structure changes in the decommissioned acid <italic>in-situ</italic> uranium mine and has positive implications for assessing the potential of natural attenuation and bioremediation strategies.</p>