AUTHOR=Wang Zong-Jie , Liu Xiaotong , Zhou Haibo , Liu Yang , Zhong Lin , Wang Xue , Tu Qiang , Huo Liujie , Yan Fu , Gu Lichuan , Müller Rolf , Zhang Youming , Bian Xiaoying , Xu Xiaokun 

TITLE=Engineering of Burkholderia thailandensis strain E264 serves as a chassis for expression of complex specialized metabolites

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1073243

DOI=10.3389/fmicb.2022.1073243

ISSN=1664-302X

ABSTRACT=<p>Heterologous expression is an indispensable approach to exploiting natural products from phylogenetically diverse microbial communities. In this study, we constructed a heterologous expression system based on strain <italic>Burkholderia thailandensis</italic> E264 by deleting efflux pump genes and screening constitutive strong promoters. The biosynthetic gene cluster (BGC) of disorazol from <italic>Sorangium cellulosum</italic> So ce12 was expressed successfully with this host, and the yield of its product, disorazol F<sub>2</sub>, rather than A<sub>1</sub>, was improved to 38.3 mg/L by promoter substitution and insertion. In addition to the disorazol gene cluster, the BGC of rhizoxin from <italic>Burkholderia rhizoxinica</italic> was also expressed efficiently, whereas no specific peak was detected when shuangdaolide BGC from <italic>Streptomyces</italic> sp. B59 was transformed into the host. This system provides another option to explore natural products from different phylogenetic taxa.</p>