AUTHOR=Tohya Mari , Otsuka Tomohiro , Yoshimoto Jiro , Ishizaki Yoichi , Kirikae Teruo , Watanabe Shin 

TITLE=Case report: Whole genome sequence of Clostridium perfringens JUM001 causing acute emphysematous cholecystitis

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1066880

DOI=10.3389/fmicb.2022.1066880

ISSN=1664-302X

ABSTRACT=<p>A strain of <italic>Clostridium perfringens</italic> was isolated from the bile sample of a patient with emphysematous cholecystitis who underwent a laparoscopic cholecystectomy, followed by treatment with meropenem and recovery. Metagenomic analysis of the bile sample showed that 99.73% of the bile microbiota consisted of <italic>C. perfringens</italic>, indicating that <italic>C. perfringens</italic> JUM001 was the causative pathogen of acute emphysematous cholecystitis in this patient. Complete genome sequencing showed that <italic>C. perfringens</italic> JUM001 contained a circular chromosome of 3,231,023 bp and two circular plasmids, pJUM001-1 of 49,289 bp and pJUM001-2 of 47,855 bp. JUM001 was found to possess a typing toxin gene, <italic>plc</italic>, but no other typing toxin genes, indicating that its toxinotype is type A. The plasmids pJUM001-1 and pJUM001-2 belonged to the pCP13-like and pCW3-like families of plasmids, respectively, which are characteristic conjugative and archetypical plasmids of <italic>C. perfringens.</italic> Phylogenetic analysis showed that JUM001 was closely related to <italic>C. perfringens</italic> strain JXNC-DD isolated from a dog in China. To our knowledge, this is the first report of whole-genome sequences of a clinical isolate of <italic>C. perfringens</italic> causing acute emphysematous cholecystitis.</p>