AUTHOR=Liu Shuang , Zhang Lei , Feng Chunlin , Zhu Jin , Li Anqi , Zhao Jingxuan , Zhang Yuan , Gao Mengdi , Shi Weina , Li Qiaoling , Zhang Xueya , Zhang Hailin , Xu Teng , Lu Junwan , Bao Qiyu 

TITLE=Characterization and Identification of a novel chromosome-encoded metallo-β-lactamase WUS-1 in Myroides albus P34

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1059997

DOI=10.3389/fmicb.2022.1059997

ISSN=1664-302X

ABSTRACT=<p>In this study, we identified and characterized a novel chromosomally-encoded class B metallo-β-lactamase (MBL) gene designated <italic>bla</italic><sub>WUS-1</sub> in a carbapenem-resistant isolate <italic>Myroides albus</italic> P34 isolated from sewage discharged from an animal farm. Comparative analysis of the deduced amino acid sequence revealed that WUS-1 shares the highest amino acid similarities with the function-characterized MBLs MUS-1 (AAN63647.1; 70.73%) and TUS-1 (AAN63648.1; 70.32%). The recombinant carrying <italic>bla</italic><sub>WUS-1</sub> exhibited increased MICs levels against a number of β-lactam antimicrobials such as carbenicillin, ampicillin and imipenem, and β-lactamase inhibitors (clavulanic acid and tazobactam). The metallo-β-lactamase WUS-1 could also hydrolyze these antimicrobials and the hydrolytic activities could be inhibited by EDTA. Genetic context analysis of <italic>bla</italic><sub>WUS-1</sub> revealed that no mobile genetic element was found in its surrounding region. The plasmid pMA84474 of <italic>Myroides albus</italic> P34 harbored 6 resistance genes (<italic>bla</italic><sub>OXA-347</sub>, <italic>aadS</italic>, <italic>bla</italic><sub>MYO-1</sub>, <italic>ereD</italic>, <italic>sul2</italic> and <italic>ermF</italic>) within an approximately 17 kb multidrug resistance (MDR) region. These genes, however, were all related to mobile genetic elements.</p>