AUTHOR=Yong Daojing , Li Yue , Gong Kai , Yu Yingying , Zhao Shuai , Duan Qiong , Ren Cailing , Li Aiying , Fu Jun , Ni Jinfeng , Zhang Youming , Li Ruijuan 

TITLE=Biocontrol of strawberry gray mold caused by Botrytis cinerea with the termite associated Streptomyces sp. sdu1201 and actinomycin D

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1051730

DOI=10.3389/fmicb.2022.1051730

ISSN=1664-302X

ABSTRACT=<p>Strawberry gray mold caused by <italic>Botrytis cinerea</italic> is one of the most severe diseases in pre- and post-harvest periods. Although fungicides have been an effective way to control this disease, they can cause serious “3R” problems (Resistance, Resurgence and Residue). In this study, <italic>Streptomyces</italic> sp. sdu1201 isolated from the hindgut of the fungus-growing termite <italic>Odontotermes formosanus</italic> revealed significant antifungal activity against <italic>B. cinerea</italic>. Four compounds (<bold>1</bold>–<bold>4</bold>) were isolated from <italic>Streptomyces</italic> sp. sdu1201 and further identified as actinomycins by the HRMS and 1D NMR data. Among them, actinomycin D had the strongest inhibitory activity against <italic>B. cinerea</italic> with the EC<sub>50</sub> value of 7.65 μg mL<sup>−1</sup>. The control effect of actinomycin D on strawberry gray mold was also tested on fruits and leaves <italic>in vitro</italic>, and its control efficiency on leaves was 78.77% at 3 d. Moreover, actinomycin D can also inhibit the polarized growth of germ tubes of <italic>B. cinerea</italic>. Therefore, <italic>Streptomyces</italic> sp. sdu1201 and actinomycin D have great potential to gray mold as biocontrol agents.</p>