AUTHOR=Ji Shunshi , Song Zexuan , Luo Lijuan , Wang Yiqian , Li Lingling , Mao Pan , Ye Changyun , Wang Yan 

TITLE=Whole-genome sequencing reveals genomic characterization of Listeria monocytogenes from food in China

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1049843

DOI=10.3389/fmicb.2022.1049843

ISSN=1664-302X

ABSTRACT=<sec><title>Introduction</title><p><italic>Listeria monocytogenes</italic> is a foodborne bacterium that could persist in food and food processing environments for a long time. Understanding the population structure and genomic characterization of foodborne <italic>L. monocytogenes</italic> is essential for the prevention and control of listeriosis.</p></sec><sec><title>Methods</title><p>A total of 322 foodborne <italic>L. monocytogenes</italic> isolates from 13 geographical locations and four food sources in China between 2000 and 2018 were selected for whole-genome sequencing.</p></sec><sec><title>Results</title><p><italic>In silico</italic> subtyping divided the 322 isolates into five serogroups, 35 sequence types (STs), 26 clonal complexes (CCs) and four lineages. Serogroup IIa was the most prevalent serogroup and ST9 was the most prevalent ST of foodborne <italic>L. monocytogenes</italic> strains isolated in China. The in-depth phylogenetic analysis on CC9 revealed that ST122 clone might be original from ST9 clone. Furthermore, 23 potentially relevant clusters were identified by pair-wised whole-genome single nucleotide polymorphism analysis, indicating that persistent- and/or cross-contamination had occurred in markets in China. ST8 and ST121 were the second and third top STs of <italic>L. monocytogenes</italic> in China, which had heterogeneity with that of <italic>L. monocytogenes</italic> isolates from other countries. The antibiotic resistance genes <italic>aacA4</italic>, <italic>tetM</italic>, <italic>tetS</italic>, <italic>dfrG</italic> carried by different mobile elements were found in <italic>L. monocytogenes</italic> strains. One lineage II strain carrying Listeria Pathogenicity Island 3 was first reported. In addition, a novel type of premature stop codon in <italic>inlA</italic> gene was identified in this study.</p></sec><sec><title>Discussion</title><p>These findings revealed the genomic characteristics and evolutionary relationship of foodborne <italic>L. monocytogenes</italic> in China on a scale larger than previous studies, which further confirmed that whole-genome sequencing analysis would be a helpful tool for routine surveillance and source-tracing investigation.</p></sec>