AUTHOR=Liao Chaosheng , Tang Xiaolong , Li Maoya , Lu Guangrou , Huang Xiaokang , Li Lin , Zhang Mingjie , Xie Yixiao , Chen Chao , Li Ping 

TITLE=Effect of lactic acid bacteria, yeast, and their mixture on the chemical composition, fermentation quality, and bacterial community of cellulase-treated Pennisetum sinese silage

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1047072

DOI=10.3389/fmicb.2022.1047072

ISSN=1664-302X

ABSTRACT=<p>The present study investigated the effects of <italic>Lentilactobacillus buchneri</italic>, <italic>Saccharomyces cerevisiae</italic>, and a mixture of the two on the cellulose degradation and microbial community of cellulase-treated <italic>Pennisetum sinese</italic> (CTPS) during biological pretreatment. The CTPS was stored without additives (CK) or with <italic>L. buchneri</italic> (L), yeast (Y, <italic>S. cerevisiae</italic>), and their mixture (LY) under anaerobic conditions for 60 days. All inoculants enhanced the anaerobic fermentation of CTPS. In relative to L, inoculations with Y and LY decreased the cellulose level of fermented-CTPS by 8.90 ~ 17.13%. Inoculation with L inhibited the growth of <italic>Weissella cibaria</italic> during anaerobic storage. However, inoculations with LY increased the relative abundance of the homofermentative bacterium <italic>Lactiplantibacillus plantarum</italic> by 6.04%. Therefore, inoculating <italic>S. cerevisiae</italic> reduced the adverse effects of <italic>L. buchneri</italic>-stimulated fermentation on cellulose degradation by altering the bacterial community during anaerobic storage of <italic>P. sinese.</italic> This work provides a new insight for the subsequent anaerobic digestion of <italic>P. sinese</italic>.</p>