AUTHOR=Dzofou Ngoumelah Daniel , Harnisch Falk , Sulheim Snorre , Heggeset Tonje Marita Bjerkan , Aune Ingvild Haugnes , Wentzel Alexander , Kretzschmar Jörg TITLE=A unified and simple medium for growing model methanogens JOURNAL=Frontiers in Microbiology VOLUME=Volume 13 - 2022 YEAR=2023 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1046260 DOI=10.3389/fmicb.2022.1046260 ISSN=1664-302X ABSTRACT=
Apart from their archetypic use in anaerobic digestion (AD) methanogenic archaea are targeted for a wide range of applications. Using different methanogenic archaea for one specific application requires the optimization of culture media to enable the growth of different strains under identical environmental conditions, e.g., in microbial electrochemical technologies (MET) for (bio)electromethanation. Here we present a new culture medium (BFS01) adapted from the DSM-120 medium by omitting resazurin, yeast extract, casitone, and using a low salt concentration, that was optimized for