AUTHOR=Yu Tianfei , Liu Xiaodong , Ai Jiamin , Wang Jiamin , Guo Yidan , Liu Xinhui , He Xiaolong , Deng Zhenshan , Jiang Yingying 

TITLE=Microbial community succession during crude oil-degrading bacterial enrichment cultivation and construction of a degrading consortium

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1044448

DOI=10.3389/fmicb.2022.1044448

ISSN=1664-302X

ABSTRACT=<p>Microbial community succession during the enrichment of crude-oil-degrading bacteria was analyzed using Illumina high-throughput sequencing to guide bacterial isolation and construction of a bacterial consortium. Community change occurred in 6 days; the most abundant phylum changed from Proteobacteria to Actinobacteria; the most abundant genera were <italic>Dietzia</italic> and <italic>unspecified_Idiomarinaceae</italic>. Two crude oil-degrading strains, <italic>Rhodococcus</italic> sp. OS62-1 and <italic>Dietzia</italic> sp. OS33, and one weak-crude-oil-degrading strain, <italic>Pseudomonas</italic> sp. P35, were isolated. A consortium comprising <italic>Rhodococcus</italic> sp. OS62-1 and <italic>Pseudomonas</italic> sp. P35 showed the highest crude-oil-degrading efficiency, reaching 85.72 ± 3.21% within 7 days, over a wide pH range (5–11) and salinity (0–80 g·L<sup>−1</sup>). Consumption of saturated hydrocarbons, aromatic hydrocarbons, and resins was greater by the consortium than by a single strain, as was degradation of short-chain-alkanes (C<sub>13</sub>–C<sub>17</sub>) according to gas-chromatography. The bacterial consortium provides technical support for bioremediation of crude oil pollution.</p>