AUTHOR=Wang Shilin , Abalori Theophilus Atio , Wang Wenhu , Deng Xiuxia , Liu Wanting , Wang Jinlan , Cao Wenxia 

TITLE=Response of soil microbial compositional and functional heterogeneity to grazing exclusion in alpine shrub and meadows in the Qinghai–Tibet Plateau

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1038805

DOI=10.3389/fmicb.2022.1038805

ISSN=1664-302X

ABSTRACT=The diversity of soil microbes modulates the functions of ecosystems, but the relationship between microbial community heterogeneity and functions has been largely overlooked. Soil microorganisms found in shrub-meadow ecosystems are highly heterogeneous and extremely sensitive to grazing. This study aimed to investigate the effect of grazing exclusion (GE) on the composition and function of microbial communities (bacteria fungi, and archaea) in 0–10 cm soils. Soil samples from heavily grazed plots (6.0 sheep/ha) and GE plots (matrix and patch areas in both) were compared. Using a combination of next-generation sequencing, vegetation features, and the associated soil property data, we provided a novel insight into the effect of GE on microbial compositional and functional heterogeneity in soils. Regarding community composition, the proportions of species in bacteria, fungi, and archaea were 97.3%, 2.3%, and 0.4%, respectively. GE significantly affected the species diversity of fungi and archaea but not that of bacteria. GE decreased the heterogeneity of bacteria (2.9% in matrix and 6.2% in patch) and archaea (31.1% in matrix and 19.7% in patch) but increased that of fungi by 1.4% in patch. Regarding community function, enzyme diversity and heterogeneity were increased by 10.4% and 9.4%, respectively, in patch after 6 years of fencing, exemplifying a high level of microbial functional redundancy. The Kyoto Encyclopedia of Genes and Genome pathways—cell growth and death, translation, digestive system, and nucleotide metabolism—were functional biomarkers (linear discriminant analysis effect size method) in matrix-non-grazed plots, whereas lipid metabolism, xenobiotics biodegradation and metabolism, and metabolism of terpenoids and polyketides, cell motility, cancer: overview, endocrine system, and membrane transport were biomarkers in patch-non-grazed plots. Additionally, GE improved the capacity for fatty acid metabolism but decreased the abundance of methane-producing archaea by 42.9%. Redundancy analysis revealed that the factors that affected microbial composition the most were soil aggregates, soil moisture, and the number of plant species, whereas those that affected microbial function the most were soil available phosphorus, soil temperature, and shrub canopy diameter. Our results quantified soil microbial heterogeneity, emphasizing the different response of the composition and function of bacteria, fungi and archaea to GE in alpine shrub and meadows.