AUTHOR=Li Xin , Zhang Wei , Zhong Xin-Ru , Han Hao-Xuan , Dong Bin 

TITLE=Genome sequencing analysis of a novel thermophilic strain Geobacillus sp. CX412

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1035311

DOI=10.3389/fmicb.2022.1035311

ISSN=1664-302X

ABSTRACT=<p>The thermophilic spore-forming strain <italic>Geobacillus</italic> sp. CX412 was isolated from hot spring soil in Tengchong City, Yunnan Province, China. We sequenced the complete genome of <italic>Geobacillus</italic> sp. CX412 using PacBio SMRT Sequencing. Genome-scale phylogenetic analysis and average nucleotide identity (ANI) results indicated that <italic>Geobacillus</italic> sp. CX412 is a novel species in the genus <italic>Geobacillus</italic>. The metabolic potential of <italic>Geobacillus</italic> sp. CX412 based on COG, KEGG, and CAZymes analysis demonstrated that <italic>Geobacillus</italic> sp. CX412 was a highly adaptable strain with an unusually high number of 73 annotated transposons in the genome, which is relatively rare in <italic>Geobacillus</italic>. Compared with the near-derived strains, it was found that <italic>Geobacillus</italic> sp. CX412 has the unique β-lactam resistance and more active metabolism (more than 50.5–100.1%). Additionally, its genome encodes glycoside hydrolases and other genes related to lignocellulose breakdown, suggesting that <italic>Geobacillus</italic> sp. CX412 has a considerable biomass degradation potential. Thus, <italic>Geobacillus</italic> sp. CX412 is a new thermophilic bacterial species that add to the increasing repertoire of known lignocellulose degraders.</p>