AUTHOR=Jiang Weiwei , Hu Dongying , Xu Yanyan , Chen Yang , Zhu Xiaoyang , Han Zhao , Ye Xin , Li Xiaojing 

TITLE=Loop-mediated isothermal amplification-microfluidic chip for the detection of Trichophyton infection

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1031388

DOI=10.3389/fmicb.2022.1031388

ISSN=1664-302X

ABSTRACT=<p><italic>Trichophyton</italic> is the most pathogenic type of fungal skin infection. It often invades and grows in a keratin-rich matrix, and lesions include human skin, hair, and fingernails (toenails). We designed LAMP primers for <italic>Trichophyton</italic> and developed a LAMP-Microfluidic chip detection system for <italic>Trichophyton</italic>. This system detects six common species of <italic>Trichophyton</italic> in the genus <italic>Trichophyton</italic>, including <italic>Trichophyton rubrum</italic>, <italic>Trichophyton mentagrophyte</italic>, <italic>Trichophyton violaceum</italic>, <italic>Trichophyton tonsurans</italic>, <italic>Trichophyton verrucosum</italic>, and <italic>Trichophyton schoenleinii</italic>. The specificity reached 100%, and the sensitivity could reach about 1 × 10<sup>2</sup> copies/μl. The entire detection process can be completed within 60 min and does not cross-react with other dermatophytes. The established LAMP-Microfluidic chip detection system has the advantages of simple operation, high specificity, and high sensitivity, and has the potential for clinical application.</p>