AUTHOR=Yang Chengxia , Han Jingyi , Berglund Björn , Zou Huiyun , Gu Congcong , Zhao Ling , Meng Chen , Zhang Hui , Ma Xianjun , Li Xuewen 

TITLE=Dissemination of blaNDM-5 and mcr-8.1 in carbapenem-resistant Klebsiella pneumoniae and Klebsiella quasipneumoniae in an animal breeding area in Eastern China

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1030490

DOI=10.3389/fmicb.2022.1030490

ISSN=1664-302X

ABSTRACT=<p>Animal farms have become one of the most important reservoirs of carbapenem-resistant <italic>Klebsiella</italic> spp. (CRK) owing to the wide usage of veterinary antibiotics. “One Health”-studies observing animals, the environment, and humans are necessary to understand the dissemination of CRK in animal breeding areas. Based on the concept of “One-Health,” 263 samples of animal feces, wastewater, well water, and human feces from 60 livestock and poultry farms in Shandong province, China were screened for CRK. Five carbapenem-resistant <italic>Klebsiella pneumoniae</italic> (CRKP) and three carbapenem-resistant <italic>Klebsiella quasipneumoniae</italic> (CRKQ) strains were isolated from animal feces, human feces, and well water. The eight strains were characterized by antimicrobial susceptibility testing, plasmid conjugation assays, whole-genome sequencing, and bioinformatics analysis. All strains carried the carbapenemase-encoding gene <italic>bla</italic><sub>NDM-5</sub>, which was flanked by the same core genetic structure (IS<italic>5</italic>-<italic>bla</italic><sub>NDM-5</sub>-<italic>ble</italic><sub>MBL</sub>-<italic>trpF</italic>-<italic>dsbD</italic>-IS<italic>26</italic>-IS<italic>Kox3</italic>) and was located on highly related conjugative IncX3 plasmids. The colistin resistance gene <italic>mcr-8.1</italic> was carried by three CRKP and located on self-transmissible IncFII(K)/IncFIA(HI1) and IncFII(pKP91)/IncFIA(HI1) plasmids. The genetic context of <italic>mcr-8.1</italic> consisted of IS<italic>903</italic>-<italic>orf</italic>-<italic>mcr-8.1-copR-baeS-dgkA</italic>-<italic>orf</italic>-IS<italic>903</italic> in three strains. Single nucleotide polymorphism (SNP) analysis confirmed the clonal spread of CRKP carrying-<italic>bla</italic><sub>NDM-5</sub> and <italic>mcr-8.1</italic> between two human workers in the same chicken farm. Additionally, the SNP analysis showed clonal expansion of CRKP and CRKQ strains from well water in different farms, and the clonal CRKP was clonally related to isolates from animal farms and a wastewater treatment plant collected in other studies in the same province. These findings suggest that CRKP and CRKQ are capable of disseminating <italic>via</italic> horizontal gene transfer and clonal expansion and may pose a significant threat to public health unless preventative measures are taken.</p>