AUTHOR=Wei Zhenlin , Xu Shuoqi , Jia Haoran , Zhang Hongmei 

TITLE=Green synthesis of silver nanoparticles from Mahonia fortunei extracts and characterization of its inhibitory effect on Chinese cabbage soft rot pathogen

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1030261

DOI=10.3389/fmicb.2022.1030261

ISSN=1664-302X

ABSTRACT=<p>The pathogenic bacterium <italic>Pectobacterium carotovorum</italic> causes soft rot in cabbage and significantly reduces plant yield. In this study, we employed <italic>Mhonia fortunei</italic> extracts to synthesis silver nanoparticles (Mf-AgNPs) and investigated their functions against <italic>P. carotovorum</italic>. The results showed that the surface plasmon resonance (SPR) peak of AgNP was 412 nm under optimal synthesis conditions. Furthermore, the results of Scanning electron microscope-Energy dispersive spectrometer (SEM-EDS) and High-resolution transmission electron microscopy (HR-TEM) revealed that the Mf-AgNPs had a spherical structure with an average diameter of 13.19 nm and the content of Ag0 ions accounted for 82.68% of the total elemental content. The X-Ray diffraction (XRD) results confirmed that AgNPs had a face-centered cubic (FCC) crystal structure, while Fourier transform infrared spectroscopy (FTIR) results indicated the presence of various biomolecules as reducing and stabilizing agents on the AgNP surface. Antibacterial activity was first evaluated by an inhibitory zone test, which revealed that 500 μg ml<sup>−1</sup> of AgNPs had antibacterial activity against <italic>P. carotovorum</italic> and four model bacteria including <italic>Staphylococcus aureus</italic>, <italic>Escherichia coli</italic>, <italic>Bacillus subtilis</italic>, and <italic>Pseudomonas aeruginosa</italic>, respectively with an antibacterial function comparable to 1 mM AgNO<sub>3</sub> solution. The Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values for <italic>P. carotovorum</italic> were 8 μg ml<sup>−1</sup>, respectively. Furthermore, AgNPs at 8 μg ml<sup>−1</sup> completely inhibited the growth of <italic>P. carotovorum</italic>, decreased their tolerance to 0.25 mM H<sub>2</sub>O<sub>2</sub> as well as considerably reduced colony formation after 1 h of treatment and thereafter. Treatment with Mf-AgNPs resulted in bacterial cell membrane destruction and biofilm formation inhibition, respectively. With an FIC (fractional inhibitory concentration) index of 0.174, AgNP and zhongshengmycin showed a significant synergistic effect. The infection of <italic>P. carotovorum</italic> to cabbage explants was significantly inhibited <italic>in vitro</italic> by a combination of 2 μg ml<sup>−1</sup> Mf-AgNP and 5 μg ml<sup>−1</sup> zhongshengmycin. In conclusion, the synthesized Mf-AgNP exhibited significant antibacterial activity against <italic>P. carotovorum</italic>.</p>