AUTHOR=Heng Pengfei , Liu Jiakai , Song Zhen , Wu Chuan , Yu Xiuzhong , He Yang 

TITLE=Rapid detection of Staphylococcus aureus using a novel multienzyme isothermal rapid amplification technique

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1027785

DOI=10.3389/fmicb.2022.1027785

ISSN=1664-302X

ABSTRACT=<p><italic>Staphylococcus aureus</italic> is a common pathogen that causes various infections. Therefore, it is crucial to develop a fast and easy detection method for diagnosing and preventing <italic>S. aureus</italic> infections. In this study, MIRA assay was developed and validated (specificity; 100%) for the detection of <italic>S. aureus</italic> with <italic>nuc</italic> as the target gene. The reaction temperature and reaction time were then optimized, and the best reaction was at 40°C, 20 min. The assay could detect <italic>S. aureus</italic> in only 25 min. Additionally, the limit of detection of MIRA was 5 × 10<sup>2</sup> CFU/ml, 10-fold lower than that of the traditional PCR. Furthermore, this assay efficiently detected 219 <italic>S. aureus</italic> of 335 strains obtained from different bacterial samples (detection accuracy; 99.40%). In conclusion, this study provides a rapid and easy-to-operate method for the detection of <italic>S. aureus</italic>, and thus can be used for the timely diagnosis and prevention of <italic>S. aureus</italic> infection.</p>