AUTHOR=Farzad Roxanna , Ha Anh D. , Aylward Frank O. 

TITLE=Diversity and genomics of giant viruses in the North Pacific Subtropical Gyre

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1021923

DOI=10.3389/fmicb.2022.1021923

ISSN=1664-302X

ABSTRACT=<p>Large double-stranded DNA viruses of the phylum <italic>Nucleocytoviricota,</italic> often referred to as “giant viruses,” are ubiquitous members of marine ecosystems that are important agents of mortality for eukaryotic plankton. Although giant viruses are known to be prevalent in marine systems, their activities in oligotrophic ocean waters remain unclear. Oligotrophic gyres constitute the majority of the ocean and assessing viral activities in these regions is therefore critical for understanding overall marine microbial processes. In this study, we generated 11 metagenome-assembled genomes (MAGs) of giant viruses from samples previously collected from Station ALOHA in the North Pacific Subtropical Gyre. Phylogenetic analyses revealed that they belong to the orders <italic>Imitervirales</italic> (<italic>n</italic> = 6), <italic>Algavirales</italic> (<italic>n</italic> = 4), and <italic>Pimascovirales</italic> (<italic>n</italic> = 1). Genome sizes ranged from ~119–574 kbp, and several of the genomes encoded predicted TCA cycle components, cytoskeletal proteins, collagen, rhodopsins, and proteins potentially involved in other cellular processes. Comparison with other marine metagenomes revealed that several have broad distribution across ocean basins and represent abundant viral constituents of pelagic surface waters. Our work sheds light on the diversity of giant viruses present in oligotrophic ocean waters across the globe.</p>