AUTHOR=Sun Rui-Ting , Zhang Ze-Zhi , Liu Ming-Yang , Feng Xiang-Cao , Zhou Nong , Feng Hai-Dong , Hashem Abeer , Abd_Allah Elsayed Fathi , Harsonowati Wiwiek , Wu Qiang-Sheng 

TITLE=Arbuscular mycorrhizal fungi and phosphorus supply accelerate main medicinal component production of Polygonum cuspidatum

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1006140

DOI=10.3389/fmicb.2022.1006140

ISSN=1664-302X

ABSTRACT=<p>The medicinal plant <italic>Polygonum cuspidatum</italic> Sieb. Et Zucc is rich in stilbenes (e.g., polygonin and resveratrol) and anthraquinones (e.g., emodin) for the therapy of human diseases, while how to increase the growth and medicinal composition concentrations of <italic>P</italic>. <italic>cuspidatum</italic> has become an urgent issue. The aim of the present study was to evaluate the effects of inoculation with an arbuscular mycorrhizal (AM) fungus, <italic>Funneliformis mosseae</italic>, on plant growth, phosphorus (P) acquisition, medicinal component concentrations, and expressions of resveratrol synthesis-associated enzyme genes of <italic>P</italic>. <italic>cuspidatum</italic> at two P levels (0 M and 0.2 M). P supply (0.2 M) stimulated root AM fungal colonization rate. <italic>F</italic>. <italic>mosseae</italic> inoculation significantly improved growth performance (height, diameter, and biomass) and root morphology (diameter, length, and projected area), irrespectively of substrate P levels. P supply and <italic>F</italic>. <italic>mosseae</italic> distinctly increased soil acid and neutral phosphatase activities, as well as root P concentrations. P supply increased root physcion and resveratrol concentrations in inoculated and uninoculated plants, along with up-regulated expressions of <italic>PcCHS1</italic>, <italic>PcCRS1</italic>, <italic>PcRS11</italic>, and <italic>PcSTS</italic>. AM plants represented significantly higher root aloe-emodin, chrysophanol, emodin, physcion, polydatin, and resveratrol concentrations than non-AM plants irrespective of P levels, coupled with up-regulated expressions of <italic>PcCHS1</italic>, <italic>PcCHS2</italic>, <italic>PcRS11</italic>, <italic>PcRS</italic>, and <italic>PcSTS</italic>. It is concluded that 0.2 M P supply and <italic>F</italic>. <italic>mosseae</italic> inoculation promoted chrysophanol, physcion, polydatin, and resveratrol concentrations of <italic>P</italic>. <italic>cuspidatum</italic>, with the increase in resveratrol associated with up-regulated expressions of related genes.</p>