AUTHOR=Chen Xin-Fei , Zhang Han , Jia Xin-Miao , Cao Jin , Li Li , Hu Xin-Lan , Li Ning , Xiao Yu-Ling , Xia Fei , Ye Li-Yan , Hu Qing-Feng , Wu Xiao-Li , Ning Li-Ping , Hsueh Po-Ren , Fan Xin , Yu Shu-Ying , Huang Jing-Jing , Xie Xiu-Li , Yang Wen-Hang , Li Ying-Xing , Zhang Ge , Zhang Jing-Jia , Duan Si-Meng , Kang Wei , Wang Tong , Li Jin , Xiao Meng , Hou Xin , Xu Ying-Chun 

TITLE=Antifungal susceptibility profiles and drug resistance mechanisms of clinical Candida duobushaemulonii isolates from China

JOURNAL=Frontiers in Microbiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1001845

DOI=10.3389/fmicb.2022.1001845

ISSN=1664-302X

ABSTRACT=<p><italic>Candida duobushaemulonii</italic>, type II <italic>Candida haemulonii</italic> complex, is closely related to <italic>Candida auris</italic> and capable of causing invasive and non-invasive infections in humans. Eleven strains of <italic>C</italic>. <italic>duobushaemulonii</italic> were collected from China Hospital Invasive Fungal Surveillance Net (CHIF-NET) and identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF), VITEK 2 Yeast Identification Card (YST), and internal transcribed spacer (ITS) sequencing. Whole genome sequencing of <italic>C</italic>. <italic>duobushaemulonii</italic> was done to determine their genotypes. Furthermore, <italic>C</italic>. <italic>duobushaemulonii</italic> strains were tested by Sensititre YeastOne™ and Clinical and Laboratory Institute (CLSI) broth microdilution panel for antifungal susceptibility. Three <italic>C</italic>. <italic>duobushaemulonii</italic> could not be identified by VITEK 2. All 11 isolates had high minimum inhibitory concentrations (MICs) to amphotericin B more than 2 μg/ml. One isolate showed a high MIC value of ≥64 μg/ml to 5-flucytosine. All isolates were wild type (WT) for triazoles and echinocandins. <italic>FUR1</italic> variation may result in <italic>C</italic>. <italic>duobushaemulonii</italic> with high MIC to 5-flucytosine. <italic>Candida duobushaemulonii</italic> mainly infects patients with weakened immunity, and the amphotericin B resistance of these isolates might represent a challenge to clinical treatment.</p>